@article{rich_sonoporation_2022, title = {Sonoporation: {Past}, {Present}, and {Future}}, volume = {7}, url = {https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/admt.202100885}, doi = {https://doi.org/10.1002/admt.202100885}, abstract = {Abstract A surge of research in intracellular delivery technologies is underway with the increased innovations in cell-based therapies and cell reprogramming. Particularly, physical cell membrane permeabilization techniques are highlighted as the leading technologies because of their unique features, including versatility, independence of cargo properties, and high-throughput delivery that is critical for providing the desired cell quantity for cell-based therapies. Amongst the physical permeabilization methods, sonoporation holds great promise and demonstrates to deliver a variety of functional cargos, such as biomolecular drugs, proteins, and plasmids, to various cells including cancer, immune, and stem cells. However, traditional bubble-based sonoporation methods usually require special contrast agents. Bubble-based sonoporation methods also have high chances of inducing irreversible damage to critical cell components, lowering the cell viability, and reducing the effectiveness of delivered cargos. To overcome these limitations, several novel non-bubble-based sonoporation mechanisms are under development. This review will cover both the bubble-based and non-bubble-based sonoporation mechanisms being employed for intracellular delivery, the technologies being investigated to overcome the limitations of traditional platforms, as well as perspectives on the future sonoporation mechanisms, technologies, and applications.}, number = {1}, journal = {Advanced Materials Technologies}, author = {Rich, Joseph and Tian, Zhenhua and Huang, Tony Jun}, year = {2022}, keywords = {acoustics, acoustofluidics, intracellular delivery, physical permeabilization, sonoporation}, pages = {2100885}, } @article{fertier_love_2009, title = {Love wave immunosensor for antibody recognition using an innovative semicarbazide surface functionalization}, volume = {140}, issn = {0925-4005}, url = {https://www.sciencedirect.com/science/article/pii/S0925400509003864}, doi = {https://doi.org/10.1016/j.snb.2009.04.069}, abstract = {A new novel and easy functionalization route of a Love wave acoustic sensor based on the α-oxo-semicarbazone bond is described. The interest is firstly to observe in real-time the immobilization of the peptide on the semicarbazide surface of the transducer and secondly to monitor the specific binding of antibodies. Site-specific immobilization of antigenic-peptides as well as binding of murine monoclonal antibodies has been shown by gravimetric measurements. A tetramethylrhodamine-labeled goat antibody directed against murine antibodies was used to further characterize the biomolecular interactions by fluorescence microscopy and surface analysis (by AFM). Our data show that the gravimetric monitoring developed from the prepared Love wave immunosensor is a promising alternative route to characterize chemical and biomolecular events.}, number = {2}, journal = {Sensors and Actuators B: Chemical}, author = {Fertier, Laurent and Cretin, Marc and Rolland, Marc and Durand, Jean-Olivier and Raehm, Laurence and Desmet, Rémi and Melnyk, Oleg and Zimmermann, Céline and Déjous, Corinne and Rebière, Dominique}, year = {2009}, keywords = {Real-time antibodies recognition monitoring, SAMs, Semicarbazide functionalization, SH-SAW, Surface modification}, pages = {616--622}, } @article{shilton_nanoliter-droplet_2014, title = {Nanoliter-{Droplet} {Acoustic} {Streaming} via {Ultra} {High} {Frequency} {Surface} {Acoustic} {Waves}}, volume = {26}, url = {https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/adma.201400091}, doi = {https://doi.org/10.1002/adma.201400091}, abstract = {The relevant length scales in sub-nanometer amplitude surface acoustic wave-driven acoustic streaming are demonstrated. We demonstrate the absence of any physical limitations preventing the downscaling of SAW-driven internal streaming to nanoliter microreactors and beyond by extending SAW microfluidics up to operating frequencies in the GHz range. This method is applied to nanoliter scale fluid mixing.}, number = {29}, journal = {Advanced Materials}, author = {Shilton, Richie J. and Travagliati, Marco and Beltram, Fabio and Cecchini, Marco}, year = {2014}, keywords = {acoustics, lab-on-a-chip, microfluidics, mixing, surface acoustic waves}, pages = {4941--4946}, } @article{ding_-chip_2012, title = {On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves}, volume = {109}, issn = {00278424}, language = {English}, number = {28}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, author = {Ding, Xiaoyun and Lin, Sz-Chin Steven and Kiraly, Brian and Yue, Hongjun and Li, Sixing and Chiang, I-Kao and Shi, Jinjie and Benkovic, Stephen J. and Huang, Tony Jun}, year = {2012}, pages = {11105--11109}, }